Influence of protein aggregation and macromolecular crowing on the dynamics and function of protiens respectively

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dc.contributor.author Homchaudhuri, Lupamudra
dc.date.accessioned 2015-09-15T14:00:45Z
dc.date.available 2015-09-15T14:00:45Z
dc.date.issued 2005
dc.identifier.other ROLL NO.004703
dc.identifier.uri http://gyan.iitg.ernet.in/handle/123456789/61
dc.description Supervisor: R. Swaminathan en_US
dc.description.abstract (Abstract Not Available) en_US
dc.description.sponsorship The phenomenon of macromolecular crowding is ubiquitous in cells. It is reported to affect equilibria and kinetics of processes taking place in cells and is also hypothesized to affect enzyme catalyzed reactions. A part of this doctoral work was directed towards ascertaining the effect of crowding on an enzyme catalyzed reaction in an environment crowded with synthetic crowding agents viz., dextran (15-500 kDa ) and Ficoll (70 and 400 kDa). The hydrolysis of p-nitrophenyl phosphate by alkaline phosphatase was used as the model reaction for the purpose.We observed that macromolecular crowding affects the catalytic rate of hydrolysis of p-nitro phenyl phosphate in a size dependent manner. In the presence of increasing concentrations of 15-70 kDa dextrans, the rates were observed to be partly transition rate limited and partly diffusion controlled. However, in the presence of the larger ... en_US
dc.language.iso en en_US
dc.relation.ispartofseries TH-0265;
dc.subject CHEMISTRY en_US
dc.title Influence of protein aggregation and macromolecular crowing on the dynamics and function of protiens respectively en_US
dc.type Thesis en_US


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